OPTIMASI DAN MODIFIKASI METODE KOLEKSI STOMATA TANAMAN KACANG PANJANG (Vigna sesquipedalis L. Fruwith) MENGGUNAKAN METODE STOMATAL PRINTING
Universitas Andalas
Indonesia
Abstract
Long beans are a popular horticultural crop known for their nutritious green vegetables, particularly the young fruit and leaves. There have been numerous studies conducted on the cultivation and breeding of long beans. However, further research is required to obtain more data on preparation of stomata imprint and morphology. This study aims to examine the optimization and modification of stomatal printing methods on long bean plants to produce imprint in a short time with clear quality. The experiment was carried out from April to June 2021 at the Plant Physiology Laboratory, Department of Agronomy, Faculty of Agriculture, Andalas University, using stomatal printing method on long bean plant leaves. The experiment consisted of two stages: first, the removal of cuticle and trichome; and second, the method of application of the printing material and the drying time of the imprint. Based on the experimental results, it was found that stomatal imprint of long bean plants could be obtained without having to remove the cuticle or trichomes. Applying a thin layer of nail polish for at least 20 minutes can produce 100% imprinted stomata, while using a thin layer of adhesive glue takes at least 10 minutes. Adhesive glue can be used as an alternative to nail polish with faster drying time and clearer imprint results in the stomatal printing method. The stomata number of long bean plants is enormous, with a density of 184-237 per mm2. The stomata type of long bean plants is parasitic.
Keywords
References
Aron Alpha. 2019. Item # AA480, ARON ALPHA Type 201. [diakses 10 Juni 2023]. < https://instantadhesives.aronalpha.net/item/aron-alpha-200-series-2/aron-alpha-200-series-general-purpose-ethyl/aa480>
Aziagba, BO., Okeke, CU., Ilodibia, CV., Ezeabara, CA., Izundu, AI., Uka, CJ. 2017. Comparative study on the epidermal features of seven varieties of Vigna unguiculata (L.) Walp cultivated in Anambra State South Eastern Nigeria. Advances in Zoology and Botany. 5(1): 4-9. DOI:10.13189/azb.2017.050102
Dahlin, RM., Brick, MA., Ogg, JB. 1992. Characterization and density of trichomes on three common bean cultivars. Economic Botany. 46(3): 299-304. DOI: 10.1007/BF02866628
Electron Microscopy Sciences. 2023. Aron Alpha® – Ethyl Ultra Speed Adhesive (Quick Bond). [diakses 10 Juni 2023]. < https://www.emsdiasum.com/aron-alpha-ethyl-ultra-speed-adhesive-quick-bond>
Fahn, A. (1992). Anatomi tumbuhan (3 ed., Vol. 2). Dalam A. Soediarto (Eds.). Gajah Mada University Press, Yogyakarta.
Fauziah, A., 'Izzah, AS. 2019. Analisis tipe stomata pada daun tumbuhan menggunakan metode stomatal printing. Di dalam: Sinergi Biologi, Sains, dan Pembelajaran untuk Menghadapi Revolusi Industri 4.0 [Internet]; Kediri, Indonesia, 21 September 2019. Kediri (ID): Prosiding Seminar Nasional HAYATI VII. 7 (1). hlm. 34-39; [diunduh 2023 Mei 20].
< https://proceeding.unpkediri.ac.id/index.php/hayati/issue/view/15/9>
Haryanti, S. (2010). Jumlah dan distribusi stomata pada daun beberapa spesies tanaman dikotil dan monokotil. Buletin Anatomi dan Fisologi. 18(2): 21-28.
Indariyani, S., Perdani, AY. 2018. Metode koleksi dan pengamatan stomata tanaman garut menggunakan pewarna kuku. Di dalam: Pengelolaan Keragaman Hayati Bertatus Konservasi (Langka, Endemik, Terancam Punah, dan Dilindungi) di luar Kawasan Konservasi [Internet]; Bandung, Indonesia, 6 Juli 2018. Surakarta (ID): Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. 4(2): hlm. 158-162; [diunduh 2021 Januari 20].
< https://smujo.id/psnmbi/issue/view/203/52>
Kusumi, K. 2013. Measuring stomatal density in rice. Bio-protocol. 3(9): 1-6. DOI: 10.21769/BioProtoc.753
Puri, V., Savla, R., Chen, K., Robinson, K., Virani, A, Michniak-Khon, B. 2022. Antifungal nail lacquer for enhanced transungual delivery of econazole nitrate. Pharmaceutics. 14 (10): 1-16. DOI: https://doi.org/10.3390/pharmaceutics
Rudall, PJ., Chen, ED., Cullen, E. 2017. Evolution and development of monocot stomata. American Journal of Botany. 104(8):1122-1141. DOI: https://doi.org/10.3732/ajb.1700086
Salisbury, FB., Ross, CW. 1995. Fisiologi tumbuhan, Edisi 4 Jilid 1. Penerbit ITB, Bandung.
Sari, DP., Harlita. 2018. Preparasi hands free section dengan teknik replika. Di dalam: Inovasi Biologi dan Pembelajarannya di Era Keterbukaan Pengetahuan [Internet]; Surakarta, Indonesia, 4 Agustus 2018. Surakarta (ID): Prosiding Seminar Nasional XV Pendidikan Biologi. 15(1): hlm. 660-664; [diunduh 2023 Mei 20]. < https://jurnal.uns.ac.id/prosbi/article/view/33036/21730>
Suhaimi. 2017. Pengaruh kadar timbal (Pb) terhadap kerapatan stomata dan kandungan klorofil pada glodokan (Polyalthia Longifolia Sonn) sebagai peneduh kota di Langsa. Elkawnie: Journal of Islamic Science and Technology. 3(1): 95-110. DOI: http://dx.doi.org/10.22373/ekw.v3i1.2755
Sundari, T., Atmaja, RP. 2011. Bentuk sel epidermis, tipe dan indeks stomata 5 genotipe kedelai pada tingkat naungan berbeda. Jurnal Biologi Indonesia. 7(1): 67-79. DOI: 10.14203/jbi.v7i1.3129
Taluta, HE., Rampea, HL., Rumondor, MJ. 2017. Pengukuran panjang dan lebar pori stomata daun beberapa varietas tanaman kacang tanah (Arachis hypogaea L.). Jurnal MIPA Unsrat Online. 6(2): 1-5. DOI: https://doi.org/10.35799/jm.6.2.
16835
Tarusu, FA., Tandi, J., Kenta, YS., Utami, IK. 2019. Uji efek ekstrak daun kacang panjang terhadap penurunan kadar glukosa darah tikus putih jantan. Farmakologika Jurnal Farmasi. 16(2): 153-167.
Program Studi Agroteknologi, Departemen Agronomi
